Touchdown rush passing7/14/2023 ![]() ![]() Albert Herrnstein holds the records for most rushing touchdowns in a season (26) and in a single game (7), having set those records for the 1902 team. Yost's "Point-a-Minute" teams from 1901 to 1904, holds the career record for rushing touchdowns with 72. Ron Johnson holds the single-game record with 347 rushing yards in a game against Wisconsin during the 1967 season. Tim Biakabutuka holds the single-season record with 1,818 rushing yards during the 1995 season. Hart also holds the career record with 1,050 carries. Michigan's career leader in rushing yards is Mike Hart with 5,040 rushing yards from 2004 to 2007. Tom Brady holds the school's record for most completions in a game, having completed 34 passes against Alabama in the 2000 Orange Bowl. Devin Gardner holds the record for passing yards in a single game (503) against Indiana in 2013. John Navarre holds the records for passing yards in a single season (3,331), set during the 2003 season. Henne also holds the career records in completions (828) and touchdown passes (87). Michigan's career leader in passing yardage is Chad Henne with 9,715 passing yards from 2004 to 2007. The Michigan Wolverines football program is a college football team that represents the University of Michigan in the National Collegiate Athletic Association's Big Ten Conference. Statistics accumulated after transferring from or before transferring to Michigan are not included here. Within those areas, the lists identify single-game, single-season and career leaders in yardage, number (receptions, rushes or passes), and touchdowns. The lists of Michigan Wolverines football statistical leaders identify individual statistical leaders of the Michigan Wolverines football program in various offensive categories, including passing, rushing, and receptions. Career leader in receiving yards, Braylon Edwards ![]()
0 Comments
![]() ![]() With the roundabout intersection control being proven as a safe, operationally efficient, and environment-friendly control treatment, a greater use of roundabouts with urban freight- roadway networks and their feasibility is analyzed in this study. Improving the efficiency at urban intersections with high truck traffic can address the freight-traffic congestion, leading to optimized goods movement as well as decreased delays, congestion, and emissions, thus enhancing the air quality in and around the communities. Intersections for urban freight-roadway networks are one of the major freight bottlenecks and are considered to be a significant contributor for congestion and delay. Freight bottlenecks are a major cause of recurring congestion which accounts for about 40% of total vehicle hours of delay in the United States. By eliminating idling at traffic lights, Carmel's roundabouts remove the equivalent of 5,000 cars' worth of carbon dioxide, while saving the city's drivers an estimated $14 million in gas annually, according to former city engineer Michael McBride.The demand for freight transportation in the United States has grown rapidly in the past couple of decades the rate at which the highway capacity is growing is not able to meet the freight-trans- portation needs, resulting in congestion and delay, ultimately affecting the users and the public with increased prices for the goods delivered, unreliable delivery times, and air-pollution concerns. "As traffic fatalities continue to rise in the U.S., we really need to use all the tools in our toolbox, and roundabouts are one of those tools that we've seen be effective," said Jessica Cicchino, vice president of research at the Insurance Institute for Highway Safety.Īnd there's an environmental benefit as well. Busy intersections, like highway exits, saw an 84% drop. The Insurance Institute for Highway Safety found that Carmel's switch to roundabouts cut injury crashes in the city by nearly half. There has been a bit of a learning curve for drivers, but Carmel has been able to remove traffic lanes even as its population has more than quadrupled. Roundabouts don't require electricity, so they continue to function during power outages and severe weather that can black out a traditional stoplight intersection. The roundabouts keep vehicles flowing, but they also slow traffic down, and slower traffic makes the roads safer for pedestrians and bicyclists. "We can keep our roads more narrow, and that's better for the environment. ![]() "We don't have to pave over paradise," Brainard said. "A roundabout would have moved 30 cars through in the same amount of time."īy 2025, Carmel, home to more than 100,000, will have just one stoplight. "Two cars are going through this intersection," he said while navigating a standard intersection. Mayor James Brainard said they make the community safer and greener.ĬBS News rode along with Brainard through some of the roundabouts, where he pointed out why he prefers traffic circles over intersections with stoplights. The city is home to 142 roundabouts and counting - more than any other city in the country. Carmel, Indiana, is sending drivers for a loop - by design. ![]() Tyler cruise sloan rider7/14/2023 ![]() ![]() He has always had a mad crush on her that has snowballed to become an obsession. If youre craving free full length XXX movies youll find them here. Fullporner is home to the best selection of free Blonde sex videos full of the hottest pornstars. Stepmother, Sloan Rider has just started dating, and her stepson, Tyler Cruise, watches her in her date night dress with envy. Similar searchestylergianna chick holdmona walesstepmomnicole ariarachael cavalliolive glasstyler cruise analkit mercersloan ryderanthony piercesloan ridertyler. Stepmom Likes It Up The Ass - Sloan Rider - Anthony Pierce & Tyler Cruise - milf anal porn hardcore performed by sloan rider on, the best full length porn site. We choose the best actors in the adult industry to give you all of their love and. Inside our playhouse you will find erotic tension-filled stories: drama, taboo, passionate romance, and nail biting sexual tension. Stepmom Likes It Up The Ass - Sloan Rider, Anthony Pierce & Tyler Cruise. get full access to MissaX About Our Taboo Playhouse. Watch HQ free lusting for stepmom – sloan rider, tyler cruise hd porn videos at Porn00. Lusting for Stepmom Sloan Rider, Tyler Cruise FullHD 1080p. It doesnt take much from Sloan to convince Anthony to drop his pants. The 44-year-old hit back at trolls and said he doesn't plan. He opted for a change of career, reinventing himself as Dallas Steele. Sloan Rider - Grandma In Love With Stepson. Jim Walker left his job as a news anchor in 2013, after 23 years. ![]() Sloan Rider - Stepmom Likes It Up The Ass. com,xnxx anime,com,xnxx sister,xnxx site:kingxxx.pro,xnxx teen,xnxx downloader,xnxx china,xnxx blacked,xnxx hot,mom xnxx,xnxx anal,blacked xnxx,https Viral,xnxx.eco,xxxindo,xxmx,pornhub,xvideoXNXX, XXX Sex,XMXX,XBXX,Xxnxx,xxx sex,sex,xnxx memek,Sex Video,Sex Porno,Seks Porno,XXX, xnxx eco, xnxx india, indian porn,xnxx,xnxx.Stepmom alison tyler Silvia Saige Sofie Marie-Stepson Fucking His Stepmom And Aunt-Porn00.mp4 Stepmom Stepmom FHot Stepmom Pops Virgin Son's Cherry & Dad Joins In The Funilthy Family – My Stepmom Fucked Out My VirginityFilthy Family – My Stepmom Fucked Out My Virginity SexMex – Horny Son Fucks His Stepmom Pamela Rios 1 stepmom anal Slimthick Vic-Stepson Gives Stepmom A Massage-Porn00.mp4 Sheena Ryder- Sheena In Stepmom Fucks Her Two Sons- Porn00.mp4 Watch Single bed temptations-Alison Tyler Krissy Lynn Sleeping Brookl.m3u8 Yasmin Scott-When Dad’s Away Stepmom Will Play!-Porn00.mp4 Stepmom milf forced Silvia Saige Sofie Marie Stepson Fucking His Stepmom And Aunt Porn00.mp4 Brazzers stepmom porn-LilHumpers-Don't Mind Him (2019) Sara Jay, Lil D-HD 1080p tyler nixon Lexi Luna lauren Phillips Nicolette Shea Alexis faux Sara jay Angela white Lena Paul Natasha nice karlee gray brooke Wylde Allison tyler Julia Ann Alexis Texas Mia Malkova Aidra fox Dillon Harper threesome foursome sauna beach Watch Single bed temptations-Alison Tyler Krissy Lynn Sleeping Brookl.m3u8 stepmom creampie carter cruise Bachelorette hardcore party outdoor message sauna soccer tennis beach big tits busty group creampie mom stepmom aunt grandma sister bratty sis creampie big tits friends movie parody Tucker Stevens Layla Larocco-After Date Threesome With Stepmom And Stepa.mp4 stepmom and son hot stepmom Son and stepmom sleep Sloan Harper-Curious Blonde Babe-Porn00.mp4 PervMom-Homemaker Stepmom Fucked in Kitchen Lauren Phillips gabbie Carter Marsha may tyler Nixon Johnny sins James Dean jordi El Niño polla erik everhard alexis Texas mia Malkova Sara jay Nikki benz Audrey bitoni peta Jensen Kendra lust Bonnie rotten christy Mack remy lecroix Lexi Luna dee Williams stepmom blackmail Stepmom and daughter lesbian Sloan rider stepmom threesome stepmom massage milf stepmom tyler tyler faith stepmom pov سكس العرب-سكس اجنبي مترجم عربي افضل الممثلين Amber Alena-آمبر ألينا 2 Kianna Dior-كينا ديور 1 Brooklyn Lee-بروكلين لي 1 Kiara Cole-كايرا كول 2 Cadey Mercury-كايدي ميركوري 1 Dava Foxx-دافا فوكس 1 lucy tyler-لوسي تايلر 1 Mackenzie Mace-ماكنزي بيريس horny stepmom sloan harper perv stepmom Shemale stepmom fucks son allison tyler Lauren Pixie-Rough Experience With Stepmom-Porn00.mp4 stepmom milf anal stepmom stepmom nuru massage Dani Daniels Dillon Harper Haley little Sara stone James deen Johnny Sins Britney Stevens Whitney Stevens Brandi love Kimmy granger clover Baltimore nyx Baltimore stepmom stepdaughter creampie threesome reverse gangbang beach Tasha reign Bambi brooks Bonn overnight with stepmom dakota tyler alyson tyler forced stepmom Videos for: Missax Lusting For Stepmom Sloan Rider Tyler Cruise. ![]() Horse thief bbq7/14/2023 ![]() ![]() They have an awesome outdoor space, hip B&W branding, delicious BBQ, and that cute bartender(Ean). Horse Thief BBQ Septemby Admin 4. Horse Thief is a total dream to decorate. 89 reviews 67 of 341 Quick Bites in Los Angeles ££ - £££ Quick Bites American Southwestern. I made some custom bunting to hang in the trees, along with a few geometric signs and a couple bright and wild floral arrangements to decorate the bar. We keep it simple, making traditional Texas style BBQ, dry rub and nothing too fancy. (oh man, i love puns) and stick with classic red, white, and blue. What stands out about Horse Thief BBQ Chef Anthony Chin, Chef & Partner: Well, we have the best brisket in LA. ![]() With just a day to throw together a few fun, high-impact decorations, I decided to shoot for the stars Meat Plates Beef Brisket 13.35 Comes with two sides. Pulled Pork Sandwich 12.35 Brioche bun, coleslaw, and BBQ sauce. Yep, that’d be the 4th of July! USA and Horse Thief share a delicious birthday. Horse Thief BBQ Sandwiches Fried Chicken Sandwich 14.40 Brioche bun, honey mustard slaw, and pickled jalapenos. Good value, good food.I was really excited to throw together some last minute first birthday decorations for the guys over at Horse Thief BBQ yesterday. Seemed to be child and dog friendly too, but keep them both on leads! All in all, the Grand Central Market is a nice spot to visit, and The Horse Thief was the highlight for me. Don't expect any table-service, fine linen or crystal glassware - it's not that sort of place. Whether youre a longtime local or just in town for GSC, wed love to see you there' / Twitter. Food is served on a tray, and you get your plastic knives and forks from the counter. Join us at the patio of Horse Thief BBQ / Golden Road Brewing in Grand Central Market (a 10 min walk from the Biltmore). There is a separate bar area that serves a range of draft beers too. I don't know if it gets rainy in LA, but if it does, this would not be a place to visit. So The Horse Thief is on the side of this market - it has no inside eating area, but communal benches outside, with parasols. It is full of food stalls selling everything from Chinese, Thai, Mexican, and Eggs (go check out Eggslut - they had queues around the block!). The location needs some explaining - it's in downtown LA's Grand Central Market, which is some sort of old market turned over to foodies. The service is fast and efficient, and the team are friendly. Horse Thief BBQ Southern BBQ in the heart of DTLA Est. The mac'n'cheese, while not something I am familiar with - I think it's an American thing - was lovely, with a perfectly good mac to cheese ratio. The pulled pork, while not spicy as I like to cook my own, was moist and tender and well seasoned. Address: 324 S Hill St (Map) Neighborhood: Downtown Cuisine: Barbeque Website: Phone: (213) Price: Pulled pork I first tried Horse Thief BBQ at The Essentials food festival in Downtown Los Angeles. ![]() The brisket was moist, had good bark (if you don't know, it's the description of the sear around the meat from the smoker) and a good smoke ring. Wildhorse Golf Course Stay & Play Pro Shop Memberships & Lessons Tournaments Clubhouse Bar & Grill 2023 Epson Tour Golf Course at Birch Creek. I had the brisket, pulled pork, mac'n'cheese and slaw, and aside from the slaw which was fairly boring, everything was excellent. I suspect there is some brisket snobbery going on with some of the write-ups that call it bland and dry - there are a lot of backyeard brisket kings in the US, and everyone has their own ideas about how it should be done. Horse Thief BBQ Restaurants Los Angeles, CALIFORNIA 15 followers Follow View all 7 employees About us Downtown Los Angeles restaurant serving central Texas inspired BBQ. I was slightly worried about the negative review about the dry brisket, but it was totally unfounded on my visit. Wednesday Movie Nights are back on Horse Thief BBQs outdoor patio Starting tomorrow, and through the month of October, well be screening classic. Horse Thief BBQ in LAs Grand Central Market joined us on Good Day LA to give us the 411 on why Texas-style BBQ is the best RECIPE. Horsethief BBQ Downtown Los Angeles Hours of Operation Friday & Saturday 11AM - 9PM Sunday - Thursday 11AM - 9PM Kitchen closes at 3:30PM. ![]() Jobsmart 125 flux core welder7/14/2023 ![]() Although the automated system shuts down the machine itself too till it cools down. Its most advantageous feature is the thermal power protection system that warns the user in case of any work overload. The voltage settings can be changed two ways as well. It can easily be operable via any standard 120v power outlet and can easily be carried out to any jobsite without hassle. The wire size happens to be between 0.030 – 0.035. Mild steel can be welded upto 18 gauge to 3/16 inches in one single pass. The wide range of materials adds to its versatility and makes it an all in one welder. Its torch can weld through stainless steel, carbon steel, aluminium, magnesium, copper, nickel, silver bronze and other types of alloys as well. For its inherent purpose, that is small work, it does pretty good for itself. It has a 20% duty cycle at 80 amps making it reliable to work at maximum strength for a maximum 2 mins. The Pro-Series MIG 125 is a reliable and high functioning welder when it comes to small to medium scale maintenance work. In-Depth Jobsmart 125 Flux Core Welder Review > Click here to See Owner Reviews on Amazon You will have access to all the components you possibly need to start off with your work, as well as the protective kit that’s needed. It’s durable duty cycle ensures maximum results increasing work efficiency and quality.Ĭherry on top of all its stellar features is that the equipment comes ready to use in a complete kit. There are few and rare such welders that can work on such a wide range of materials. The qualities and features of this welder are par excellence, this light weight equipment has a strong compact body that gives way to streamlined functions. What makes Jobsmart 125 Flux Core Welder better than the competition? Why should you buy Jobsmart 125 Flux Core Welder?.In-Depth Jobsmart 125 Flux Core Welder Review.> Click here to See Owner Reviews on Amazon.What makes Jobsmart 125 Flux Core Welder better than the competition?.We’re sure that by the end of this review you’ll be entirely convinced of its merits. You’ll see what features make it unique to other welders in the market and understand all its unique positive and negative aspects. ![]() ![]() Our review will cover all integral aspects that must be known to prospective buyers. Moreover, the entire equipment comes ready to use with all the components you need to get started right away! The varieties of steel can be welded up to 18 gauge to 3/16 inches in a single pass. The Pro-Series MMIG 125 amp fluxcore welder has a 20% of duty cycle at about 80amps which makes it operate for 2 mins straight without a single glitch at maximum speed before needing to cool down the welder. Its lightweight and compact design makes it the ideal go-to equipment for work around the house or perhaps a farm.Īny welding task that involves carbon steel and various other alloys can easily be covered with this 125 amp welder. The Jobsmart MIG 125 is the ultimate small scale equipment that covers all your basic maintenance necessities. ![]() Pro player fortnite key bindings7/14/2023 ![]() ![]() ![]() Nvidia Reflex Low Latency: Try On or On+ Boost, there's a difference depending on your PC. Show FPS: Off, but you can keep it On when testing the settings. Medium: Builds from 360M and loot from 268M Low View Distance: See builds from 270M and 200M This means Medium the best option for view distance because it still allows you to see loot from a far distance. Instead it does have an impact on how far you can see builds and loot. Changing view distance doesn't actually change how far you can see enemies. Anything lower than 70 and characters look like a worldstar fight that looks like it was recorded on the first camera ever created. You can go down to 1600x900 if your PC is lower end.Ĭolor Blind Mode: Find a mode and strength that lets you see better in storm or in general.ģD Resolution: 100%, can lower this if your PC is lower end. But, I preferred capped because it feels more stable. This unlocks settings that you can change that you normally wouldn't be able to on Performance Mode.įPS Limit: Capped if Nvidia Reflex Off, Uncapped if it is On. r/FortniteMobile - Fortnite: Battle Royale Mobile Useful Linksįortnite Home Page Fortnite Stats TrackerĪtlantis Scrims EU Discord Atlantis Scrims NA Discord NA East Open Scrims Discord NA West Open Scrims Discord EU Open Scrims Discord FNPL NA Scrims NA West High IQ Scrims (NAW Prac Opens) Cano ScrimsįN Mature Boomercord Fortnite over 40 Adult Scrims User ResourcesĬreative List of Practice Courses MegathreadĬonsole Only Tips Megathread (An extension of From Bot To Tryhard Sweat)īefore doing anything, change your Render Mode to DX11 and then restart your game. r/Fortnite - Fortnite: Save the World /r/FortniteBR - Fortnite: Battle Royale /r/FortniteCreative - Fortnite: Creative For a full list of rules, please check the Subreddit Rules Page before submitting! Following Reddiquette is required! Related Subreddits Users are expected to behave maturely and respectfully, stay on topic, and don't spoil results for 24 hours. This is the developer-supported subreddit that is tailored for those who want to keep up to date on the pro scene, tournaments, competitive plays and figure out new tips/tricks on how to play the current meta.Ĭlick here to view the latest patch notes. ![]() Fortnite: Battle Royale is a free-to-play Battle Royale game mode developed by Epic Games. ![]() ![]() Unfortunately, we could not test this, as it is not available to utilize until the program is registered.Īside from disabling the password feature, the program's trial limits you to only one account, but we were still able to get a clear picture of how well the program works. The program also offers a helpful way to keep your finances private by applying a password to access your checkbook. Users can monitor deposits and payments without worrying about mathematical errors, since Checkbook does all the hard work for them. All we had to do was fill in the appropriate fields with the date, check title and amount, and the account balances itself out. ![]() Balancing our books was delightfully simple. There is also an excellent step-by-step Help file to guide anyone who is not perfectly comfortable with the program. The entry fields are intuitive and should not be a problem to navigate. The program's interface is reminiscent of the inside of a checkbook. Utilizing a familiar design and garnering fantastic results, this may be a dream come true for those who dread balancing their checkbooks each month. are registered as futures commission merchants with the CFTC and are members of the NFA.Checkbook offers users a chance to keep their finances in order with this simple program. and Merrill Lynch Professional Clearing Corp. and Merrill Lynch Professional Clearing Corp., both of which are registered broker-dealers and Members of SIPC, and, in other jurisdictions, by locally registered entities. Trading in securities and financial instruments, and strategic advisory, and other investment banking activities, are performed globally by investment banking affiliates of Bank of America Corporation ("Investment Banking Affiliates"), including, in the United States, BofA Securities, Inc. Lending, other commercial banking activities, and trading in certain financial instruments are performed globally by banking affiliates of Bank of America Corporation, including Bank of America, N.A., Member FDIC. "Bank of America" and "BofA Securities" are the marketing names used by the Global Banking and Global Markets divisions of Bank of America Corporation. Investment products offered by Investment Banking Affiliates: are registered as futures commission merchants with the CFTC and members of the NFA. Trading in securities and financial instruments, and strategic advisory, and other investment banking activities, are performed globally by investment banking affiliates of Bank of America Corporation (“Investment Banking Affiliates”), including, in the United States, BofA Securities, Inc. Lending, other commercial banking activities, and trading in certain financial instruments are performed globally by banking affiliates of Bank of America Corporation, including Bank of America, N.A. “Bank of America” and “BofA Securities” are the marketing names used by the Global Banking and Global Markets divisions of Bank of America Corporation. Or download Global Card Access from major app stores, including: ![]() We’re here to make business faster, smarter, and more secure. No matter which device you’re using, this all-in-one platform was designed to make key account information available whenever, wherever, and however You can also set up custom alerts, which help monitor your account activity, and text, email, or call from us can help save precious time and give you peace of mind.īoth options mean greater control, security, and convenience. We make it simple with the Lock and Unlock feature if your card is ever lost. ![]() Let’s work together to improve your card’s safety. If you forget your PIN, you can view it or change it from any mobile device.Īlerts available in North America with planned expansion to EMEA and APAC View PIN available in North America, EMEA, India and AustraliaĬhange PIN available in North America through mobile-friendly website with planned expansion to EMEA and APAC. Plus your monthly statements are now at your fingertips. Your mobile-friendly dashboard gives you a clear view of important account details: credit limit, balance, available credit, and recent card activity. View the Global Card Access dashboard online or within the GCA app. Managing your corporate card just got a lot easier. ![]() Tidal logo psd7/13/2023 ![]() Find your way with voice navigation and turn-by-turn driving, transit, and walking directions. Quickly type or write it on your desktop, and you can even sync across devices. ![]() Grocery lists, home improvement projects, books to read-use a Sticky Note to help you remember. Free for commercial use High Quality Images You can find & download the most popular Tidal Vectors on Freepik. 4,000+ Vectors, Stock Photos & PSD files. Image License: Personal Use Only DMCA Report Download Problems. Find & Download Free Graphic Resources for Tidal. Tidal Logo vector,Tidal icon Download as SVG,transparent, png, psd, pdf Ai ,vector free. Resolution: 1629x1553 Size: 580 KB Downloads: 1 Views: 8 Image type: PNG Contributor: Send Message. See what’s newĬoordinate schedules, plan family game night-with the Calendar app 3 you can easily see what’s on the agenda and stay a step ahead. Cartoon Painted Comic Tidal Png And Psd - Illustration, Transparent Png Download. Stay on top of your day with the Mail and Calendar apps. , Gmail, iCloud, and Yahoo!-they're all in one place, making it easy to send messages and schedule appointments with your most important people. ![]() Not only does it come with a great set of editing tools, but it also has a smart search function, and convenient ways to share 5 your photos with friends and family. Each user can extract the tracks of interest and create their own chain, a personal playlist of their favorite music. The Tidal logo is the prototype of an impressive information base. We have 181 free Tidal Image logo png, vector logos, logo templates and icons. Update: Tidal Logo PNG The emblem is like a mosaic from which any figure can be laid out. ![]() Microsoft Photos has everything you need to keep your digital memories organized. Search results for Tidal Image logo png icon vector. Are you looking for Tidal logo text effect used for your banner, poster and other design project Pngtree offers 10000+ editable Tidal logo font png, psd for you. ![]() Toast 11 titanium mac free download7/13/2023 ![]() ![]() The biggest problem, however with this app, is its price. There are very few apps out there, in my opinion that are better at these tasks than Toast. Pros: Easy audio and video capturing and editingĬonclusion: Toast Titanium is THE audio and video editing and capture application for your PC or Mac. You can convert for enjoyment on iPad, iPhone, or your favorite on-the-go-device with just a few clicks. Ripping audiobook CDs to iTunes, with chapters, pause/resume support and variable playback speeds is also easy with Toast. Toast's audio filters and tools help you clean up your audio and then burn it to CD or DVD, or send to iTunes for syncing with your iDevice. You can digitize and restore your LP's and tapes, without the pops, clicks and hisses. Toast can even send it automatically to iTunes for syncing with your favorite iDevice. You can save streaming video from your favorite web sites and then burn the content to DVD for viewing on your big-screen TV. ![]() Toast's MusicID automatically adds artist and title tags to captured music for easy syncing to your iTunes library.Ĭapturing streaming web video is just as easy with Toast. You can capture any streaming Internet audio on your Mac, without interference from other apps or system sounds. You can trim start and end points, remove unwanted segments in the middle of video clips before export, or even apply audio effects. With it, you can create stunning Blu-ray video or HD DVD discs for playback on compatible set top players as well as your PC. You can use just about any HD video source. Toast is the fastest way to capture video from your AVCHD camcorder, with no lengthy imports. It's one of the premier multimedia tools available for your Mac. That's one of the reasons why I really like Toast Titanium. Having a lot of multimedia on your PC usually means you're going to need a tool to help you capture and preserve it. Having a Mac usually means having a lot of multimedia on your PC. Take total control of multimedia on your Mac with this very cool tool. ![]() Redox western blot technique7/13/2023 ![]() ![]() Expression of TRX in mammalian cells is enhanced by a variety of stresses such as H 2O 2, x-ray and UV exposure, or anticancer drug treatment ( 4, 9, 10, 11, 12). Subsequent studies have shown that TRX is released from several cell types through a leaderless pathway in response to various stimulations ( 6, 7), and that APCs release TRX to provide the reducing environment for the activation of T lymphocytes ( 8). Human TRX was originally cloned as a soluble factor produced by human T cell leukemia virus type I (HTLV-I)-transformed ATL2 cells ( 3, 4) or as an autocrine growth factor produced by EBV-transformed B cells ( 5). Thioredoxin (TRX) 3 is a small (12 kDa) ubiquitous protein containing a conserved active site, -Cys-Gly-Pro-Cys-, and plays a variety of redox-related roles in organisms ranging from Escherichia coli to humans ( 1, 2). These results collectively suggest that the oxidative stress-induced TRX release from T lymphocytes depends on a redox-sensitive event and may be regulated by negative feedback loops using reactive oxygen species-mediated signal transductions. Confocal microscopy and Western blot analysis show that extracellular rTRX-WT added to the culture does not obviously enter T lymphocytes until 24 h. Preincubation of the transfectant with rTRX-WT for 1 h at 37☌, but not 0☌, results in a significant suppression of the TRX release, reactive oxygen species, and caspase-3 activity induced by H 2O 2, respectively. H 2O 2-induced release of TRX from the transfectant is inhibited by the presence of rTRX-WT in a dose-dependent manner. In contrast, another type of transfectant expressing the tagged TRX mutant (C32S/C35S CS) fails to release the protein. In the culture supernatant of a Jurkat transfectant expressing the tagged TRX-wild type (WT), the tagged TRX protein is rapidly released at 1 h and kept at a constant level until 6 h after the addition of H 2O 2. The level of TRX release is augmented upon the addition of H 2O 2, but suppressed upon the addition of N-acetylcysteine. Human T cell leukemia virus type I-transformed T lymphocytes constitutively release a large amount of TRX. We show here that a redox-active site in TRX is essential for its release from T lymphocytes in response to H 2O 2 and extracellular TRX regulates its own H 2O 2-induced release. Thioredoxin (TRX) is released from various types of mammalian cells despite no typical secretory signal sequence. ![]() |